Troubleshoot needed for sample syringe error message

<p> Hi!</p><p></p><p>I'm trying to start a sample set that contains some injections using injection volume  100 uL (previous injections in the same sample set used 10 uL). System is giving an error and the error message reads:</p><p></p><p>General failure: not enough volume left in the sample syringe.</p><p></p><p>I guess that maybe something has been set up in the instrument  (UPLC Acquity, the original binary system) but I don't know what or where to check it. Thanks a lot in advance!!</p><p></p><p>Dorcas</p>

Answers

  • Hello

    Is this the exact message? Were there a series or errors, before this. This i a little non-specific, I want to confirm that this is the message and that it is the sample syringe that the issue is related to.

    Please check the these items first can you confirm that there enough needlewash solvent. Then we need to check the the size of the loop and the syringe configured.

    Let me know.

    Thank you

    Liz

  • Also can you confirm the injection mode used. This was a sugestion from our Global upport Manager.

    Thank you,

    Liz