Hi, We have a method that requires that our samples are stored in the autosampler at 7deg +/- 3 deg. As we put on our run the temperature in the lab was approaching 30deg. the sample cooler was reading approx 9deg. Although 9 deg falls within the allowed range for the method we found that the we could not put on the run unless we changed the method to 9 deg. The sample cooler seemed unable to cool below the 9 degrees. Even at this as the temperature in the lab climbed the run aborted shortly after started as the sample temperature could not be maintained by the instrument. My questions are: At what temperature (in the laboratory) will the sample cooler not be able to maintain the cooling of the sample chamber? Why won't the UPLC run when the sample temperature is within the range of the method? We have noticed this occur on several occasions.Thanks for your help on this.