Determining dwell volume for UPLC
How have other people determined the dwell volume for their Acquity systems? Should I base the flow rate on a minimum backpressure or just choose a default flow rate? What about the ramp for the gradient? One reference I read mentioned a 20 minute gradient. How should I address the two different slopes observed in the data during a gradient run? Initially, it rises steeply, then flattens out a bit and remains steady for the remainder of the gradient change. Which slope do I draw the tangent on?