Empower data points
Hi ,
Can you display raw data (acquired) points in a chromatogram ?
I can only tabulate points across peak or 'enable tick marks' (not entirely sure what this is for) but not show data points on baseline plot
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Hi ,
Can you display raw data (acquired) points in a chromatogram ?
I can only tabulate points across peak or 'enable tick marks' (not entirely sure what this is for) but not show data points on baseline plot
Answers
thanks
Currently most our LC's are default with 'Peak width : >0.1min (2 s resp time (5Hz)), though I am struggling to see how 'enable tick marks' shows these individual points and corresponds to points across peak (assuming increments in this case 0.20 seconds?)
Ok ,, not what I expected
Would be nice to actually see where data points are collected for peak integration by Apex track, especially low level response and for sensitivity
Only option as attached (if 0.2 increments correct using UV setting?)
this gives theoretical data points ?
Assuming you align the peak start/end settings properly in the view and use the correct increments for your data rate (so a 0.2 sec setting would be for a 5 pt/sec data rate), those vertical hashmarks are your actual data points that are used. The algorithm would be calculating the slope between each of those to determine if it is a peak and where the beginning/end of the peak are based on your integration settings.
See attached
Top right example displaying sample points (auto I believe) on another CDS and identifying small peak (algorithm as described in previous post)
I'm not fully understanding where vertical hashmarks are and use of increments
You can set this between 0.00 - 39354 ?
First, you probably need to tweak your "seconds" value. A setting of 10 seconds would mean your IM's sampling rate (in order to match the actual datapoints) would have to be 0.1 pts/sec...which is clearly not the case based on that baseline. So what is your actual sampling rate? I've attached an example from an analysis.
Then, to help visualize if you need it, you can zoom in a bit more. You can also change the color and thickness of the hashmarks if you need to...under the plot properties' Colors tab...Tick Marks/Fractions. Note that it seems as if you need to go really big (I had to go at least to a thickness of 20+ to get much of a visual change). Maybe set them to something like red to contrast the black of the baseline to help see them?