Air sensor calibration out of range
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Hi everyone,
Just did a lot of work on a very problematic nanoAcquity for a professor. What seems like the last issue: after replacing the needle, Mass Lynx wouldn't let me load a method until I characterize the needle volume. The needle is 26 uL. I selected 30 and ran the characterization. It gave me an error that the needle calibration was out of range (about 24). So I went into service mode and tried calibrating the air sensor. That, also was out of range. Visibly, everything looks good. I can also see the bubble moving in and out of the black box of the sensor when it is running the calibration. One thought I had is that this is the nanoSM but the computer recognises the normal Sample Manager. Are the volumes typically different?
Everything else seems to be working, I just need to either fix or work around this volume issue.
Thanks,
Dean
Just did a lot of work on a very problematic nanoAcquity for a professor. What seems like the last issue: after replacing the needle, Mass Lynx wouldn't let me load a method until I characterize the needle volume. The needle is 26 uL. I selected 30 and ran the characterization. It gave me an error that the needle calibration was out of range (about 24). So I went into service mode and tried calibrating the air sensor. That, also was out of range. Visibly, everything looks good. I can also see the bubble moving in and out of the black box of the sensor when it is running the calibration. One thought I had is that this is the nanoSM but the computer recognises the normal Sample Manager. Are the volumes typically different?
Everything else seems to be working, I just need to either fix or work around this volume issue.
Thanks,
Dean
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Best Answer
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Thank you for your question. Due to the technical nature of your question, if you are still having issues, please contact iRequest Technical Service/ Support: http://www.waters.com/waters/iRequest-Technical-Support/nav.htm?cid=10006027
A Waters Support Specialist will contact you to quickly address your needs. Thank you.
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Answers
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If I may add: I see conflicting information about the actually calibration of the volume detector. Usually the needle should be screwed into port 3 of the rheodyne, and the VDD to Port 2. When calibrating the VDD, am I supposed to switch these, as it may seem from reading other posts on here?0
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Hi Dean,
You need to calibrate the air sensor (Volume Detection Device) but that calibration requires a service code. So you'll need to contact Waters Technical Service. 888-292-8377
-Patrick0 -
Hi Patrick,
I have the service code. I found it written down. However, when I calibrate the air sensor, it says 'out of range' and tells me it is something like 24, which seems reasonable to me. Is there a step to calibrating it that I am missing? Do I have to switch the positions of any connections on the rheodyne?
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