Difference between numbers for the curve effects

Wouter
Wouter
in Instrumentation and Mass Spectrometry
Could someone explain me what is the difference between curve effect number 1 (Immediately goes to the specified conditions) and number 11 (Maintains start conditions until mext step).
What start condition is ment here? Is 1 en 11 not exactely the same thing?

Best Answer

  • MJS
    MJS
    Answer ✓
    It means that the gradiënt 3.0 / 97.0 pumps until the time 1.70, last second?
    Yes.  At 1.2 min, it is pumping at 3/97.  With a curve of 11, it just stays at 3/97 until the next step at 1.7 min.  At 1.7 min, it makes an immediate jump to 95/5.

    When there was a number 1 curve it still pumps 3.0 / 97.0 until the time is 1.70, but not last second?
    Sorry for the confusion, but no.  If you were using a curve of 1, the jump is at the beginning of the segment at 1.2 min.  When the injection reaches 1.2 min, it makes an immediate jump to 95/5.

    So the "segment" is the two rows.  A value of 1 makes the change at the beginning between them at the first possible second, an 11 makes the change at the end at the last possible second.  The values 2 through 10 make a more gradual change rather than a full 100% step.  A value of 6 is most linear and smooth.

Answers

  • MJS
    MJS
    Essentially, both start at the initial conditions of the step in the gradient.  A 1 would see an immediate, 100% change where 11 would see it delayed until the last possible second between the two rows of the gradient table.  See page 3-4 of the attached help file for the curve parameter.  It has a handy graphic.

    Generally everyone uses a 6 for most applications.  I'll use a 1 or 11 during a shutdown portion of an IM or during a cleaning cycle scenario.
  • Wouter
    Wouter
    Thank you, Still don't see it. 

    Tijd

    Flow rate (ml/min)

    % Mobiele fase 1 (A)

    % Mobiele fase 2 (B)

    Curve

    1.20

    0,8

    3,0

    97,0

    11

    1.70

    0,8

    95,0

    5,0

    11

    It means that the gradiënt 3.0 / 97.0 pumps until the time 1.70, last second?   When there was a number 1 curve it still pumps 3.0 / 97.0 until the time is 1.70, but not last second??


  • Wouter
    Wouter
    OK great , it's absolutly cristalclear. thank you

Categories