# Separate or combined prime

I have a question regarding Alliance systems.

Does it matter if I (wet) prime each line separately or if I do it combined (4x 25% composition) if the total volume is the same?

So, is 5 [email protected] 5ml/min prime for 100%A only (and then 100% for each remaining channel; total volume 25 ml*4 channels = 100ml) equal to [email protected] 5ml/min for 25/25/25/25 A/B/C/D? (100ml combined)?

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## Answers